Cell, 2002-10, Vol.111 (2), p.197-208
2002
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- Autor(en) / Beteiligte
- Titel
- Histone Methyltransferase Activity of a Drosophila Polycomb Group Repressor Complex
- Ist Teil von
- Cell, 2002-10, Vol.111 (2), p.197-208
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2002
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Polycomb group (PcG) proteins maintain transcriptional repression during development, likely by creating repressive chromatin states. The Extra Sex Combs (ESC) and Enhancer of Zeste [E(Z)] proteins are partners in an essential PcG complex, but its full composition and biochemical activities are not known. A SET domain in E(Z) suggests this complex might methylate histones. We purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU(Z)12. A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3. Mutations in the E(Z) SET domain disrupt methyltransferase activity in vitro and HOX gene repression in vivo. These results identify E(Z) as a PcG protein with enzymatic activity and implicate histone methylation in PcG-mediated silencing.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/S0092-8674(02)00976-5Titel-ID: cdi_proquest_miscellaneous_72643510
- Format
- –
- Schlagworte
- Animals, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins - genetics, Drosophila - embryology, Drosophila - metabolism, Drosophila Proteins - genetics, Drosophila Proteins - isolation & purification, Drosophila Proteins - metabolism, Gene Silencing, Histone Methyltransferases, Histone-Lysine N-Methyltransferase, Homeodomain Proteins, Insect Proteins - isolation & purification, Lysine - metabolism, Methyltransferases - metabolism, Molecular Chaperones, Nuclear Proteins - isolation & purification, Nuclear Proteins - metabolism, Polycomb Repressive Complex 1, Polycomb Repressive Complex 2, Protein Methyltransferases, Recombination, Genetic, Repressor Proteins - isolation & purification, Repressor Proteins - metabolism, Retinoblastoma-Binding Protein 4, Transcription Factors