Details

Autor(en) / Beteiligte

• Nair, Satish K.
• Christianson, David W.

Titel

Structural properties of human carbonic anhydrase II at pH 9.5

Ist Teil von

• Biochemical and biophysical research communications, 1991-12, Vol.181 (2), p.579-584

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1991

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The structure of human carbonic anhydrase II at pH 9.5 has been studied by X-ray crystallographic methods to 2.2 Å resolution. These studies complement those performed under acidic conditions in which the catalytically-important proton-shuttle group, His-64, exhibits conformational mobility about side-chain torsion angle χ 1. However, no structural changes are observed in the conformation of His-64 at high pH. Therefore, we conclude that the protonation of His-64 (as well as zinc-bound hydroxide) may be a factor which contributes to the predominantly “out” conformation for His-64 observed at low pH.