Details

Autor(en) / Beteiligte

• O'Harte, Finbarr
• Burcher, Elizabeth
• Lovas, Sandor
• Smith, D. David
• Vaudry, Hubert
• Conlon, J. Michael

Titel

Ranakinin: A Novel NK1 Tachykinin Receptor Agonist Isolated with Neurokinin B from the Brain of the Frog Rana ridibunda

Ist Teil von

• Journal of neurochemistry, 1991-12, Vol.57 (6), p.2086-2091

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• An extract of the whole brain of the frog Rana ridibunda contained high concentrations of substance P‐like immunoreactivity, measured with an antiserum directed against the COOH‐terminal region of mammalian substance p and neurokinin b‐like immurtoreactivity, measured with an antiserum directed against the NH2‐terminus of neurokinin B. The primary structure of the substance p‐related peptide (ranakinin) was established as: Lys‐Pro‐Asn‐Pro‐Glu‐Arg‐Phe‐Tyr‐Gly‐Leu‐Met‐NH2. Mammalian substance P was not present in the extract. The primary structure of the neurokinin b‐related peptide was established as: Asp‐Met‐His‐Asp‐Phe‐Phe‐Val‐Gly‐Leu‐Met‐NH2. This amino acid sequence is the same as that of mammalian neurokinin B. Ranakinin was equipotent with substance p and [Sar9,‐Met(O2)11]substance p in inhibiting the binding of 125I‐Bolton‐Hunter‐[Sar9, Met(O2)11]substance p, a selective radio‐ligand for the NK1 receptor, to binding sites in rat subman‐dibular gland membranes (IC50 1.6 ± 0.3 nM; n = 5). It is concluded that ranakinin is a preferred agonist for the mammalian NK1 tachykinin receptor subtype.