Life sciences (1973), 1991, Vol.49 (23), p.1755-1760
1991
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Evidence for platelet-activating factor receptors in several B lymphoblastoid cell lines
- Ist Teil von
- Life sciences (1973), 1991, Vol.49 (23), p.1755-1760
- Ort / Verlag
- Amsterdam: Elsevier Inc
- Erscheinungsjahr
- 1991
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Previous studies have shown that Raji, an Epstein-Barr virus (EBV)-immortalized Burkitt lymphoma B cell line, contains functional platelet-activating factor (PAF) receptors. Twelve other lymphoid cell lines, including Burkitt and non-Burkitt B cell lines, T cell lines, and a non B, non T cell line were tested for the presence of PAF receptors. Radioligand binding studies conducted at 4 °C revealed that six lymphoid cell lines of B cell origin (Raji, P3HR-1, BJAB, BJA/HR-1, Dakiki and PB-1) could specifically bind [ 3H]PAF. Treatment of four (Raji, P3HR-1, Dakiki and PB-1) of the above lymphoid cell lines with PAF resulted in an increase in free intracellular calcium, indicating that these specific PAF binding sites were functional PAF receptors. Other B cell lines (Daudi, B95-8, sfBT, CB-1), T cell lines (MOLT-4, CCRF-CEM) and a non B, non T cell line (NALM-6) had no PAF binding sites and showed no PAF-induced increase in intracellular calcium levels. These studies demonstrate evidence for the presence of PAF receptors on several B lymphocyte cell lines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0024-3205eISSN: 1879-0631DOI: 10.1016/0024-3205(91)90318-6Titel-ID: cdi_proquest_miscellaneous_72455503
- Format
- –
- Schlagworte
- Animals, B-Lymphocytes - chemistry, B-Lymphocytes - metabolism, Binding Sites, Biological and medical sciences, Calcium - metabolism, Cell Line, Fundamental and applied biological sciences. Psychology, Fundamental immunology, Humans, Immunobiology, Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation, membrane proteins, Platelet Activating Factor - metabolism, Platelet Activating Factor - pharmacology, Platelet Membrane Glycoproteins, platelet-activating factor, Receptors, Cell Surface - analysis, Receptors, Cell Surface - metabolism, Receptors, G-Protein-Coupled, Tumor Cells, Cultured