Molecular cell, 2001-12, Vol.8 (6), p.1243-1254
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Acetylation of p53 Activates Transcription through Recruitment of Coactivators/Histone Acetyltransferases
- Ist Teil von
- Molecular cell, 2001-12, Vol.8 (6), p.1243-1254
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2001
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- Cellular DNA damage causes stabilization and activation of the tumor suppressor and transcription factor p53, in part by promoting multiple covalent modifications of the p53 protein, including acetylation. We investigated the importance of acetylation in p53 function and the mechanism by which acetylation influences p53 activity. Acetylation site substitutions reduced p53-dependent transcriptional induction and G1 cell cycle arrest. Chromatin immunoprecipitation analysis of the endogenous p21 promoter showed increased association of p53, coactivators (CBP and TRRAP), and acetylated histones following cell irradiation. Results with acetylation-defective p53 demonstrate that the critical function of acetylation is not to increase the DNA binding affinity of p53 but rather to promote coactivator recruitment and histone acetylation. Therefore, we propose that an acetylation cascade consisting of p53 acetylation-dependent recruitment of coactivators/HATs is crucial for p53 function.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/S1097-2765(01)00414-2Titel-ID: cdi_proquest_miscellaneous_72392763
- Format
- –
- Schlagworte
- Acetylation, Acetyltransferases - metabolism, Adaptor Proteins, Signal Transducing, Amino Acid Substitution - genetics, CBP protein, Cell Cycle, Cell Cycle Proteins, Chromatin - chemistry, Chromatin - genetics, Chromatin - metabolism, Chromatography, Gel, CREB-Binding Protein, Cyclin-Dependent Kinase Inhibitor p21, Cyclins - genetics, histone acetyltransferase, Histone Acetyltransferases, Histones - chemistry, Histones - metabolism, Humans, Mutation - genetics, Nuclear Proteins - metabolism, p300-CBP Transcription Factors, Precipitin Tests, Promoter Regions, Genetic - genetics, Protein Binding, RNA, Messenger - genetics, RNA, Messenger - metabolism, Saccharomyces cerevisiae Proteins, Trans-Activators - metabolism, Transcription Factors, Transcription, Genetic, Transcriptional Activation, TRRAP protein, Tumor Cells, Cultured, Tumor Suppressor Protein p53 - chemistry, Tumor Suppressor Protein p53 - genetics, Tumor Suppressor Protein p53 - metabolism