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BibTeX
Hippocampal mossy fibers induce assembly and clustering of PSD95-containing postsynaptic densities independent of glutamate receptor activation
Journal of comparative neurology (1911), 2001-11, Vol.440 (3), p.284-298
Qin, Lixuan
Marrs, Glen S.
McKim, Ross
Dailey, Michael E.
2001
Details
Autor(en) / Beteiligte
Qin, Lixuan
Marrs, Glen S.
McKim, Ross
Dailey, Michael E.
Titel
Hippocampal mossy fibers induce assembly and clustering of PSD95-containing postsynaptic densities independent of glutamate receptor activation
Ist Teil von
Journal of comparative neurology (1911), 2001-11, Vol.440 (3), p.284-298
Ort / Verlag
New York: John Wiley & Sons, Inc
Erscheinungsjahr
2001
Link zum Volltext
Quelle
Wiley HSS Collection
Beschreibungen/Notizen
Factors that regulate the formation, spatial patterning, and maturation of CNS synapses are poorly understood. We used organotypic hippocampal slice cultures derived from developing (P5–P7) rat to test whether synaptic activity regulates the development and organization of postsynaptic structures at mossy fiber (MF) giant synapses. Antibodies to a prominent postsynaptic density (PSD) scaffold protein, PSD95, identified large (>1 μm) and irregularly shaped PSD assemblies that codistributed with synapsin‐I or metabotropic glutamate receptor 7b (mGluR7b) ‐immunolabeled MF terminals in area CA3. To investigate the spatial organization of synaptic PSDs on individual pyramidal cells, neurons in slice cultures were transfected with a vector encoding a GFP‐PSD95 fusion protein. Confocal three‐dimensional reconstructions revealed clusters of PSDs along proximal dendrites of transfected pyramidal neurons in area CA3, but not in CA1. Clusters averaged 7.6 μm in length (range, 2.2–29 μm) and contained up to 35 individual PSDs (mean, 8.3). PSD clusters failed to form when slices were cultured without MFs, indicating that MFs induce cluster assembly. Chronic blockade of N‐methyl‐D‐apartate– and AMPA/kainate‐type glutamate receptors did not disrupt MF targeting or de novo formation of PSD clusters with a normal distribution on target cells. Additionally, glutamate receptor blockers did not alter the ultrastructural development of MF giant synapses containing multiple puncta adherens‐like junctions and asymmetric synaptic junctions at dendritic shaft and spine domains, respectively. The results indicate that MF axons can induce the assembly and clustering of PSD95‐containing postsynaptic complexes, displaying a normal subcellular and tissue distribution, by mechanisms that are independent of ionotropic glutamate receptor activation. J. Comp. Neurol. 440:284–298, 2001. © 2001 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9967
eISSN: 1096-9861
DOI: 10.1002/cne.1386
Titel-ID: cdi_proquest_miscellaneous_72362471
Format
–
Schlagworte
Animals
,
Dendrites - metabolism
,
GFP
,
hippocampal slice
,
Hippocampus - cytology
,
Hippocampus - metabolism
,
In Vitro Techniques
,
mossy fibers
,
Mossy Fibers, Hippocampal - physiology
,
Mossy Fibers, Hippocampal - ultrastructure
,
Nerve Tissue Proteins - metabolism
,
postsynaptic density 95 protein
,
Pyramidal Cells - metabolism
,
Rats
,
Receptors, Glutamate - physiology
,
Synapses - physiology
,
Synapses - ultrastructure
,
synaptic activity
,
synaptogenesis
,
thorny excrescence
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