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Circadian Regulation of Diverse Gene Products Revealed by mRNA Expression Profiling of Synchronized Fibroblasts
Ist Teil von
The Journal of biological chemistry, 2001-12, Vol.276 (50), p.46751-46758
Ort / Verlag
United States: American Society for Biochemistry and Molecular Biology
Erscheinungsjahr
2001
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
Genes under a 24-h regulation period may represent drug targets relevant to diseases involving circadian dysfunctions. As
a testing model of the circadian clock system, we have used synchronized rat fibroblasts that are known to express at least
six genes in a circadian fashion. We have determined the expression patterns of 9957 transcripts every 4 h over a total period
of 76 h using high density oligonucleotide microarrays. The spectral analysis of our mRNA profiling data indicated that â¼2%
(85 genes) of all expressed genes followed a robust circadian pattern. We have confirmed the circadian expression of previously
known clock or clock-driven genes, and we identified 81 novel circadian genes. The majority of the circadian-regulated gene
products are known and are involved in diverse cellular functions. We have classified these circadian genes in seven clusters
according to their phase of cycling. Our pathway analysis of the mRNA profiling data strongly suggests a direct link between
circadian rhythm and cell cycle.