Details

Autor(en) / Beteiligte

• Grundschober, C
• Delaunay, F
• Pühlhofer, A
• Triqueneaux, G
• Laudet, V
• Bartfai, T
• Nef, P

Titel

Circadian Regulation of Diverse Gene Products Revealed by mRNA Expression Profiling of Synchronized Fibroblasts

Ist Teil von

• The Journal of biological chemistry, 2001-12, Vol.276 (50), p.46751-46758

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• Genes under a 24-h regulation period may represent drug targets relevant to diseases involving circadian dysfunctions. As a testing model of the circadian clock system, we have used synchronized rat fibroblasts that are known to express at least six genes in a circadian fashion. We have determined the expression patterns of 9957 transcripts every 4 h over a total period of 76 h using high density oligonucleotide microarrays. The spectral analysis of our mRNA profiling data indicated that ∼2% (85 genes) of all expressed genes followed a robust circadian pattern. We have confirmed the circadian expression of previously known clock or clock-driven genes, and we identified 81 novel circadian genes. The majority of the circadian-regulated gene products are known and are involved in diverse cellular functions. We have classified these circadian genes in seven clusters according to their phase of cycling. Our pathway analysis of the mRNA profiling data strongly suggests a direct link between circadian rhythm and cell cycle.