Details

Autor(en) / Beteiligte

• Petinaki, E
• Kontos, F
• Miriagou, V
• Maniati, M
• Hatzi, F
• Maniatis, A.N

Titel

Survey of methicillin-resistant coagulase-negative staphylococci in the hospitals of central Greece

Ist Teil von

• International journal of antimicrobial agents, 2001-12, Vol.18 (6), p.563-566

Ort / Verlag

London: Elsevier B.V

Erscheinungsjahr

2001

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• A sample of 450 consecutive, non-replicated coagulase-negative staphylococci (CoNS), collected from clinical specimens during the period 2000–2001 from the five major hospitals of Thessaly district (Central Greece) were investigated for resistance to methicillin. Most of the isolates had been collected in a sporadic fashion from the intensive care units and the surgical wards of the participating hospitals. The majority of the isolates (76%) were Staphylococcus epidermidis (50%), Staphylococcus haemolyticus (14.8%) and Staphylococcus hominis (11.1%). All 316 isolates (70%) were classified as resistant according to NCCLS breakpoints (MIC≥0.5 mg/l); 268 (59.5%) of them were mecA-positive in a PCR-based assay. All isolates with MIC≥8 mg/l carried the gene, while, only 23.8% of isolates with MIC, 0.5–4 mg/l were carriers. Only 9% of the mecA-positive isolates were found to be sensitive to various non-β-lactams, while 41.8% of the isolates were resistant to more than three antimicrobial groups apart from β-lactams. Molecular typing by PFGE showed apparent heterogeneity among isolates of each species and the absence of predominant clones.