Details

Autor(en) / Beteiligte

• MICHAUD, SOPHIE
• MENARD, SUZANNE
• GAUDREAU, CHRISTIANE
• ARBEIT, ROBERT D

Titel

Comparison of SmaI-defined genotypes of Campylobacter jejuni examined by KpnI: a population-based study

Ist Teil von

• Journal of medical microbiology, 2001-12, Vol.50 (12), p.1075-1081

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2001

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• *Centre Hospitalier Universitaire de Sherbrooke and Direction de la Santé Publique de l'Estrie, Sherbrooke, Centre Hospitalier de l'Université de Montreal, Hôpital St-Luc, Montréal, Québec, Canada; Research Service, VA Medical Center, and Department of Medicine, Boston University School of Medicine, Boston, MA, USA Corresponding author: Dr S. Michaud (e-mail: smichaud{at}courrier.usherb.ca ). Received 20 March 2000; revised manuscript received 22 Feb. 2001; accepted 16 May 2001. Abstract Pulsed-field gel electrophoresis (PFGE) was used to analyse 147 isolates collected in two regions of Québec province (Estrie and Montréal) between March 1998 and Feb. 1999, to determine the utility of molecular strain typing for a population-based collection of Campylobacter jejuni and to compare directly the discriminatory power of Sma I and Kpn I restriction digests. With a combination of epidemiological criteria including space and time plus molecular strain typing, 49% of isolates from Estrie and 39% of isolates from Montréal were identified as belonging to a putative cluster. For 41% of the cases, sources were either missing or explicitly unknown; the remaining sources were subject to recall bias. Thus, the evaluation of sporadic cases of campylobacter enteritis by descriptive clinical investigation alone is neither sensitive nor reliable for identifying sources of infection. In the PFGE analysis, Kpn I digests provided appreciably greater discriminatory power than Sma I digests. When combining the PFGE analyses with basic epidemiological criteria, 30% of the putative Sma I clusters were inconsistent with the epidemiological criteria compared with 17% of the Kpn I clusters. Among the 98 isolates assigned to clusters by Sma I, only 65% gave concordant results with Kpn I. In contrast, among the 81 isolates assigned to clusters by Kpn I, 92% gave concordant results with Sma I. Finally, clusters that were epidemiologically related to ingestion of raw milk and specific water sources correlated better with the typing results based on Kpn I than Sma I. Thus, Kpn I is the enzyme of choice for molecular epidemiology studies of C. jejuni . The combination of continuous epidemiological surveillance and molecular strain typing may be useful for identifying new sources and mechanisms of transmission for community-acquired C. jejuni infection and ultimately for developing new approaches to prevention.