Details

Autor(en) / Beteiligte

• Masui, T
• Hosotani, R
• Tsuji, S
• Miyamoto, Y
• Yasuda, S
• Ida, J
• Nakajima, S
• Kawaguchi, M
• Kobayashi, H
• Koizumi, M
• Toyoda, E
• Tulachan, S
• Arii, S
• Doi, R
• Imamura, M

Titel

Expression of METH-1 and METH-2 in Pancreatic Cancer

Ist Teil von

• Clinical cancer research, 2001-11, Vol.7 (11), p.3437-3443

Ort / Verlag

United States: American Association for Cancer Research

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• Purpose: METH-1/hADAMTS-1 and METH-2/hADAMTS-8 are recently identified genes that inhibit angiogenesis, and the murine homologue, ADAMTS-1, shows metalloproteinase function. Because the significance of METH-1 and METH-2 has not been determined in solid tumors, we examined the mRNA expressions of these molecules in pancreatic cancer and hepatocellular carcinoma (HCC). Experimental Design: METH-1 and METH-2 mRNA expressions were identified in six pancreatic cancer cell lines and were quantified by TaqMan reverse transcription-PCR in 18 paired samples of pancreatic cancer and surrounding noncancerous pancreas, and in 14 samples of pancreatic cancer. METH-1 mRNA expression was also examined in 16 pairs of HCC and cirrhotic liver. Vascularity was estimated by CD34 staining. The correlation between clinicopathological factors and METH-1 expression was additionally analyzed. Results: Four of six pancreatic cancer cell lines expressed METH-1, and 1/6 expressed METH-2. METH-1 was substantially expressed in both pancreatic cancer and noncancerous pancreas, but METH-2 was not. METH-1 expression in pancreatic cancer tissue was significantly lower than that in noncancerous pancreas ( P = 0.002), and a similar result was obtained between HCC and cirrhotic liver ( P = 0.003). METH-1 expression did not show a significant correlation with vascularity in pancreatic cancer or in HCC. However, pancreatic cancer with higher expression of METH-1 showed significantly severe lymph node metastasis or retroperitoneal invasion ( P = 0.033 and P = 0.018, respectively) and worse prognosis ( P = 0.038). Conclusions: METH-1, which was initially reported to have a potent antiangiogenic effect, does not seem to be a predominant determinant of tumor vascularity in pancreatic cancer. Rather, METH-1 seems to be involved in progression of pancreatic cancer through local invasion and lymph node metastasis.