FEBS letters, 2001-11, Vol.507 (3), p.327-330
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Kinetic study of the processing by dipeptidyl-peptidase IV/CD26 of neuropeptides involved in pancreatic insulin secretion
- Ist Teil von
- FEBS letters, 2001-11, Vol.507 (3), p.327-330
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2001
- Quelle
- Elektronische Zeitschriftenbibliothek
- Beschreibungen/Notizen
- Dipeptidyl-peptidase IV (DPPIV/CD26) metabolizes neuropeptides regulating insulin secretion. We studied the in vitro steady-state kinetics of DPPIV/CD26-mediated truncation of vasoactive intestinal peptide (VIP), pituitary adenylyl cyclase-activating peptide (PACAP27 and PACAP38), gastrin-releasing peptide (GRP) and neuropeptide Y (NPY). DPPIV/CD26 sequentially cleaves off two dipeptides of VIP, PACAP27, PACAP38 and GRP. GRP situates between the best DPPIV/CD26 substrates reported, comparable to NPY. Surprisingly, the C-terminal extension of PACAP38, distant from the scissile bond, improves both PACAP38 binding and turnover. Therefore, residues remote from the scissile bond can modulate DPPIV/CD26 substrate selectivity as well as residues flanking it.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(01)02982-9Titel-ID: cdi_proquest_miscellaneous_72256382
- Format
- –
- Schlagworte
- CD26, Dipeptidyl Peptidase 4 - metabolism, Dipeptidyl-peptidase IV, DPP, dipeptidyl-peptidase, Gastrin-releasing peptide, Gastrin-Releasing Peptide - metabolism, GIP, glucose-dependent insulinotropic peptide, GLP, glucagon-like peptide, GRP, gastrin-releasing peptide, Humans, Insulin - metabolism, Insulin Secretion, Kinetics, Mass Spectrometry, Neuropeptide Y, Neuropeptide Y - metabolism, Neuropeptides - metabolism, NPY, neuropeptide Y, PACAP, pituitary adenylyl cyclase-activating peptide, Pancreas - metabolism, Pituitary Adenylate Cyclase-Activating Polypeptide, Pituitary adenylyl cyclase-activating peptide, Substrate Specificity, Vasoactive intestinal peptide, Vasoactive Intestinal Peptide - metabolism, VIP, vasoactive intestinal peptide