Details

Autor(en) / Beteiligte

• Oshima, Taku
• Aiba, Hirofumi
• Masuda, Yasushi
• Kanaya, Shigehiko
• Sugiura, Masahito
• Wanner, Barry L.
• Mori, Hirotada
• Mizuno, Takeshi

Titel

Transcriptome analysis of all two‐component regulatory system mutants of Escherichia coli K‐12

Ist Teil von

• Molecular microbiology, 2002-10, Vol.46 (1), p.281-291

Ort / Verlag

Oxford, UK: Blackwell Science, Ltd

Erscheinungsjahr

2002

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Summary We have systematically examined the mRNA profiles of 36 two‐component deletion mutants, which include all two‐component regulatory systems of Escherichia coli, under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria–Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two‐component systems. A high correlation coefficient of expression profile was found between several two‐component mutants. Together, these results support the view that a network of functional interactions, such as cross‐regulation, exists between different two‐component systems. The compiled data are avail‐able at our website (http:ecoli.aist‐nara.ac.jpxpanalysis 2components).