Details

Autor(en) / Beteiligte

• MACLENNAN, A. J
• BRECHA, N
• KHRESTCHATISKY, M
• STERNINI, C
• TILLAKARATNE, N. J. K
• CHIANG, M.-Y
• ANDERSON, K
• LAI, M
• TOBIN, A. J

Titel

Independent cellular and ontogenetic expression of mRNAS encoding three α polypeptides of the rat GABAA receptor

Ist Teil von

• Neuroscience, 1991, Vol.43 (2-3), p.369-380

Ort / Verlag

Oxford: Elsevier

Erscheinungsjahr

1991

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Previous studies have shown that several distinct but related polypeptides can serve as alpha subunits of functional GABAA receptors. Furthermore, the diversity of these polypeptides at least partially accounts for the functional heterogeneity of GABAA receptors. In this paper, we report the results of in situ hybridization studies using probes derived from our recently reported cDNAs for alpha 1, alpha 2, and alpha 4 GABAA receptor polypeptides. We show that the mRNAs that encode these isoforms have distinct regional and cellular distributions and are present at widely varying levels within the rat brain. In addition, our Northern blot analyses indicate that each of these three alpha mRNAs has a distinct pattern of ontogenetic regulation. Differential regulation of alpha polypeptide isoforms may lead to changes in GABAA receptor function during ontogeny as well as to distinct cellular responses to GABA and GABA-related drugs.