Analytical biochemistry, 1991-05, Vol.195 (1), p.74-76
1991
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Details
- Autor(en) / Beteiligte
- Titel
- Isolation of DNA from blood
- Ist Teil von
- Analytical biochemistry, 1991-05, Vol.195 (1), p.74-76
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1991
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation. This isolation method readily adapts to multiple samples. The DNA is characterized by high yield, solubility, lack of protein contamination, and ease of restriction endonuclease digestion.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1016/0003-2697(91)90297-7Titel-ID: cdi_proquest_miscellaneous_72086429
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Animals, Biological and medical sciences, Cattle, Cell Nucleus - chemistry, Chemical Precipitation, DNA - blood, DNA - isolation & purification, DNA Restriction Enzymes, Dna, deoxyribonucleoproteins, Ethanol, Fundamental and applied biological sciences. Psychology, Male, Nucleic acids, Pronase, Spermatozoa - chemistry