Details

Autor(en) / Beteiligte

• Shaw, P. W.
• Fuller, B. J.
• Bernard, A.
• Shaw, R. W.

Titel

Vitrification of mouse oocytes: Improved rates of survival, fertilization, and development to blastocysts

Ist Teil von

• Molecular reproduction and development, 1991-08, Vol.29 (4), p.373-378

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1991

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Rall and Fahy's (1985) vitrification procedure for the cryopreservation of 8‐cell embryos was applied to unfertilized mouse oocytes. Unchanged, this method resulted in a mean of 24.1% of vitrified oocytes fertilizing and developing to blastocysts in vitro. Exposure of oocytes to the cryoprotectant media, but without the vitrification, resulted in 30.8% developing to blastocysts. Modifications to the durations of and media used in the dilution and equilibration steps of the procedure produced a final protocol giving a mean of 55.4% of vitrified oocytes and 72.4% of nonvitrified VS1‐exposed oocytes developing to blastocysts; 85.7% of control oocytes develop to blastocysts. Osmotically induced damage was found to be the most important cause of loss of viability in these methods. Cooling of oocytes to 5–8°C during the procedure had no significant effect on their viability. No parthenogenetic activation of oocytes occurred as a result of exposure to the procedure.