Details

Autor(en) / Beteiligte

• Bañó, M. Carmen
• Cohen, Smadar
• Visscher, Karyn B
• Allcock, Harry R
• Langer, Robert

Titel

A Novel Synthetic Method for Hybridoma Cell Encapsulation

Ist Teil von

• Bio/technology (New York, N.Y. 1983), 1991-05, Vol.9 (5), p.468-471

Ort / Verlag

New York, NY: Nature Publications

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• We report here what we believe is the first example of the encapsulation of hybridoma cells within a synthetic polymer by a simple gelation with dissolved cations in water, and at room temperature. Two lines of hybridoma cells were encapsulated within calcium cross-linked polyphosphazene gel microbeads without affecting their viability or their capability to produce antibodies. Interaction of these gel beads with the positively-charged polyelectrolyte, poly(L-lysine), of 102-kD molecular weight, produced a semipermeable membrane that was capable of retaining the cell-secreted antibodies inside the beads. Cell density increased 3.5-fold within 13 days concomitant with a 6.4-fold increase in antibody production. These synthetic membranes have the potential to aid in protein recovery schemes.