Details

Autor(en) / Beteiligte

• Hotez, Peter J
• Ashcom, James
• Bin, Zhan
• Bethony, Jeffrey
• Williamson, Angela
• Hawdon, John M
• Jianjun, Feng
• Dobardzic, Azra
• Rizo, Ivania
• Bolden, Janelle
• Jin, Qun
• Yan, Wang
• Dobardzic, Reshad
• Chung-Debose, Sophia
• Crowell, Melissa
• Datu, Bennett
• Delaney, Angela
• Dragonovski, Dilyan
• Jiang, Yang
• Yueyuan, Liu
• Ghosh, Kashinath
• Loukas, Alex
• Brandt, Walter
• Russell, Philip K
• Zook, Bernard C

Titel

EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE

Ist Teil von

• The Journal of parasitology, 2002-08, Vol.88 (4), p.684-690

Ort / Verlag

Lawrence, KS: American Society of Parasitologists

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D–like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 ± 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5–18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastrointestinal tract.