Details

Autor(en) / Beteiligte

• Gorczynski, Reginald
• Chen, Zhiqi
• Kai, Yu
• Lee, Lydia
• Wong, Simon
• Marsden, Philip A

Titel

CD200 Is a Ligand for All Members of the CD200R Family of Immunoregulatory Molecules

Ist Teil von

• The Journal of immunology (1950), 2004-06, Vol.172 (12), p.7744-7749

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2004

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand. We have also cloned and sequenced a family of CD200Rs, but identify an amino terminus to two of the three isoforms not recognized by previous researchers. We show by FACS, using FITC-labeled CD200Fc, that COS7 cells transfected with all CD200R isoforms bind CD200 as ligand, although the functional consequences of this binding likely differs between the different isoforms. mAbs directed against the CD200 R1/R4 isoforms altered IL-2/IL-4 cytokine production and suppressed CTL responses in a fashion comparable to CD200Fc, with a significantly lesser effect seen following addition of anti-CD200 R2/R3.