Biotechnology letters, 2004-04, Vol.26 (7), p.575-580
2004
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- Autor(en) / Beteiligte
- Titel
- Functional identification of the gene locus Ncg12319 and characterization of catechol 1,2-dioxygenase in Corynebacterium glutamicum
- Ist Teil von
- Biotechnology letters, 2004-04, Vol.26 (7), p.575-580
- Ort / Verlag
- Dordrecht: Springer
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Corynebacterium glutamicum assimilated phenol, benzoate, 4-hydroxybenzoate p-cresol and 3,4-dihydroxybenzoate. Ring cleavage was by catechol 1,2-dioxygenase when phenol or benzoate was used and by protocatechuate 3,4-dioxygenase when the others were used as substrate. The locus ncg12319 of its genome was cloned and expressed in Escherichia coli. Enzyme assays showed that ncg12319 encodes a catechol 1,2-dioxygenase. This catechol 1,2-dioxygenase was purified and accepted catechol, 3-, or 4-methylcatechols, but not chlorinated catechols, as substrates. The optimal temperature and pH for catechol cleavage catalyzed by the enzyme were 30 degrees C and 9, respectively, and the Km and Vmax were determined to be 4.24 micromol l(-1) and 3.7 micromol l(-1) min(-1) mg(-1) protein, respectively.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0141-5492eISSN: 1573-6776DOI: 10.1023/b:bile.0000021958.86258.08Titel-ID: cdi_proquest_miscellaneous_71972744
- Format
- –
- Schlagworte
- Amino Acid Sequence, Bacteria, Biological and medical sciences, Biotechnology, Catechol 1,2-Dioxygenase, Chromosome Mapping, Cloning, Molecular - methods, Corynebacterium - enzymology, Corynebacterium - genetics, Corynebacterium glutamicum, Dioxygenases - chemistry, Dioxygenases - genetics, Dioxygenases - metabolism, E coli, Enzyme Activation, Enzyme Stability, Escherichia coli, Escherichia coli - enzymology, Escherichia coli - genetics, Fundamental and applied biological sciences. Psychology, Gene Expression Profiling, Gene loci, Molecular Sequence Data, Molecular Weight, Phenols, Protein Conformation, Proteins, Recombinant Proteins - chemistry, Recombinant Proteins - metabolism, Sequence Homology, Amino Acid