Details

Autor(en) / Beteiligte

• Gronthos, S.
• Brahim, J.
• Li, W.
• Fisher, L.W.
• Cherman, N.
• Boyde, A.
• DenBesten, P.
• Robey, P. Gehron
• Shi, S.

Titel

Stem Cell Properties of Human Dental Pulp Stem Cells

Ist Teil von

• Journal of dental research, 2002-08, Vol.81 (8), p.531-535

Ort / Verlag

United States: SAGE Publications

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.