Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
A Leishmania donovani gene that confers accelerated recovery from stationary phase growth arrest
Ist Teil von
International journal for parasitology, 2004-06, Vol.34 (7), p.803-811
Ort / Verlag
Oxford: Elsevier Ltd
Erscheinungsjahr
2004
Quelle
MEDLINE
Beschreibungen/Notizen
We have isolated a gene, LdGF1, from the protozoan parasite
Leishmania donovani. Overexpression of this gene confers a strong selective advantage in liquid culture after stationary phase growth arrest. We could show that recombinant
L. donovani or
Leishmania major, when overexpressing
LdGF1, recover faster from a stationary phase growth arrest than control parasite strains. While no advantage of
LdGF1 overexpression could be observed in log phase cultures or after a hydroxyurea-induced S-phase growth arrest, recovery from a cell cycle arrest due to serum deprivation was faster in
LdGF1-overexpressing strains. This was found to be due to an accelerated release from a G
1 cell cycle arrest. By contrast, in a BALB/c mouse infection system, overexpression of
LdGF1 in
L. major resulted in reduced virulence. We conclude that increased levels of LdGF1 are beneficiary during recovery from G
1 cell cycle arrest, but pose a disadvantage inside a mammalian host. These results are discussed in the context of the observed loss of virulence during in vitro passage of
Leishmania parasites.