Journal of plant physiology, 2004-04, Vol.161 (4), p.397-403
2004
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- Autor(en) / Beteiligte
- Titel
- Osmotic induced stimulation of the reduction of the viability dye 2,3,5-triphenyltetrazolium chloride by maize roots and callus cultures
- Ist Teil von
- Journal of plant physiology, 2004-04, Vol.161 (4), p.397-403
- Ort / Verlag
- Jena: Elsevier GmbH
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Live cells can reduce colorless 2,3,5-triphenyltetrazolium chloride (TTC) to a red insoluble compound, formazan. Maize ( Zea mays) callus, when osmotically stressed by 0.53 mol/L mannitol, produced 7-times or more formazan than untreated control callus. This result was seen with all osmotica tested and could not be attributed to differences in TTC uptake rate or accumulation, increased respiration rate as measured by O 2 uptake, or to de novo protein synthesis. Increased formazan production could be detected after 2.5 h of exposure to osmotic stress and leveled off after 48 h of exposure. The increased formazan production was only detected when callus was moved from high osmotic medium to low osmotic, TTC-containing medium. Abscisic acid increased TTC reduction only when added in combination with 0.53 mol/L mannitol. Incubation of maize seedling roots with 0.53 mol/L mannitol also increased formazan production as seen visually. Further studies are needed to determine the cause of the increased formazan production. These results show that TTC viability measurements must be carefully evaluated with appropriate controls to confirm their validity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0176-1617eISSN: 1618-1328DOI: 10.1078/0176-1617-01237Titel-ID: cdi_proquest_miscellaneous_71909377
- Format
- –
- Schlagworte
- 2,3,5-triphenyltetrazolium chloride, abscisic acid, azo compounds, Biological and medical sciences, Biotechnology, callus, Coloring Agents - metabolism, corn, culture media, Culture Techniques, duration, Eukaryotic cell cultures, formazan, Formazans - metabolism, Fundamental and applied biological sciences. Psychology, grain crops, In vitro propagation: entire plant regeneration from tissues and cell cultures, Mannitol - pharmacology, Methods. Procedures. Technologies, Osmotic Pressure, Osmotic stress, Oxidation-Reduction - drug effects, Plant cells and fungal cells, plant proteins, plant response, Plant Roots - drug effects, Plant Roots - metabolism, plant stress, reduction, root tips, Tetrazolium Salts - metabolism, Time Factors, tissue culture, transcription (genetics), translation (genetics), triphenyltetrazolium chloride, viability test, water content, water stress, Zea mays, Zea mays - drug effects, Zea mays - metabolism, Zea mays callus, Zea mays roots