Details

Autor(en) / Beteiligte

• Yermolaieva, Olena
• Leonard, A. Soren
• Schnizler, Mikael K.
• Abboud, Francois M.
• Welsh, Michael J.

Titel

Extracellular Acidosis Increases Neuronal Cell Calcium by Activating Acid-Sensing Ion Channel 1a

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2004-04, Vol.101 (17), p.6752-6757

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• Acid-sensing ion channel (ASIC) 1a subunit is expressed in synapses of central neurons where it contributes to synaptic plasticity. However, whether these channels can conduct Ca2+ and thereby raise the cytosolic Ca2+ concentration, [ Ca2+] c, and possibly alter neuronal physiology has been uncertain. We found that extracellular acidosis opened ASIC1a channels, which provided a pathway for Ca2+ entry and elevated [ Ca2+] c in wild-type, but not ASIC1-/-, hippocampal neurons. Acid application also raised [ Ca2+] c and evoked Ca2+ currents in heterologous cells expressing ASIC1a. Although ASIC2a is also expressed in central neurons, neither ASIC2a homomultimeric channels nor ASIC1a/2a heteromultimers showed H+-activated [ Ca2+] c elevation or Ca2+ currents. Because extracellular acidosis accompanying cerebral ischemia contributes to neuronal injury, we tested the effect of acidosis on cell death measured as lactate dehydrogenase release. Eliminating ASIC1a from neurons or treating ASIC1a-expressing cells with the ASIC blocker amiloride attenuated acidosis-induced cell injury. These results indicate that ASIC1a provides a non-voltage-gated pathway for Ca2+ to enter neurons. Thus, it may provide a target for modulation of [ Ca2+] c.