Details

Autor(en) / Beteiligte

• Peterson, Kenneth R.
• Fedosyuk, Halyna
• Zelenchuk, Lesya
• Nakamoto, Betty
• Yannaki, Evangelia
• Stamatoyannopoulos, George
• Ciciotte, Steven
• Peters, Luanne L.
• Scott, Linda M.
• Papayannopoulou, Thalia

Titel

Transgenic Cre expression mice for generation of erythroid-specific gene alterations

Ist Teil von

• Genesis (New York, N.Y. : 2000), 2004-05, Vol.39 (1), p.1-9

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

2004

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Transgenic mice that express Cre recombinase in erythroid cell lineages were developed so that genes affecting erythropoiesis/hematopoiesis may be altered without necessarily affecting fetus viability. A micro‐LCR cassette‐β‐globin promoter‐Cre recombinase gene (μLCR‐βpr‐Cre) construct was synthesized and used to generate transgenic mice. Concurrently, we produced mice containing a μLCR‐loxP‐flanked β sickle gene (μLCR‐loxP‐βS‐loxP) construct. μLCR‐βpr‐Cre mice with intact transgenes in variable copy number were identified. Cre expression was assessed by RNAse protection and RT‐PCR. Cre function was ascertained by breeding to μLCR‐loxP‐βS‐loxP mice. We demonstrate that βS expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP‐flanked βS gene was restricted to erythroid cell lineages. genesis 39:1–9, 2004. © 2004 Wiley‐Liss, Inc.