Vaccine, 2004-05, Vol.22 (15), p.1873-1885
2004
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Details
- Autor(en) / Beteiligte
- Titel
- Intracellular fate and immunogenicity of B. subtilis spores
- Ist Teil von
- Vaccine, 2004-05, Vol.22 (15), p.1873-1885
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2004
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- To support our work on the development of bacterial spores as oral vaccines we examined the immunogenicity and intracellular fate of Bacillus subtilis endospores in a murine model. Mice dosed orally with spores developed systemic IgG and mucosal sIgA responses. Analysis of IgG subclasses revealed a predominance of the IgG2a subclass during the early stages of immunisation. Analysis of cytokine mRNA in GALT and lymphoid organs showed early induction of IFN-γ, a Th1 cytokine, as well as the pro-inflammatory cytokine TNF-α. Significant levels of IgG antibodies were produced against vegetative bacilli following dosing with spores. This showed that spores could germinate in the GI tract. In vitro studies detailing the intracellular fate and persistence of spores in a macrophage-like cell line (RAW264.7) demonstrated that spores could germinate efficiently in macrophages, initiate gene expression as well as inducing pro-inflammatory cytokines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2003.11.021Titel-ID: cdi_proquest_miscellaneous_71894779
- Format
- –
- Schlagworte
- Animals, Antigen-Presenting Cells - immunology, Applied microbiology, Bacillus subtilis, Bacillus subtilis - immunology, Bacillus subtilis - ultrastructure, Bacterial Proteins - immunology, Bacterial Vaccines - immunology, Biological and medical sciences, Cytokines - biosynthesis, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Fundamental and applied biological sciences. Psychology, Immunity, Mucosal - immunology, Lac Operon - genetics, Lymphoid Tissue - microbiology, Macrophages, Macrophages - immunology, Macrophages - microbiology, Macrophages - ultrastructure, Mice, Mice, Inbred C57BL, Microbiology, Microscopy, Confocal, Microscopy, Electron, Persistence, Rabbits, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - biosynthesis, RNA, Messenger - immunology, Spores, Spores, Bacterial - immunology, Spores, Bacterial - ultrastructure, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)