Brain research, 2004-05, Vol.1009 (1), p.234-237
2004
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- Autor(en) / Beteiligte
- Titel
- Excitatory effects of ATP on rat dorsomedial hypothalamic neurons
- Ist Teil von
- Brain research, 2004-05, Vol.1009 (1), p.234-237
- Ort / Verlag
- London: Elsevier B.V
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca 2+ ([Ca] i). The rise in [Ca] i was dependent on external Ca 2+ and Na +, was blocked by Ca 2+ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca 2+ channels, resulting in an increase in [Ca] i.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-8993eISSN: 1872-6240DOI: 10.1016/j.brainres.2004.03.001Titel-ID: cdi_proquest_miscellaneous_71888890
- Format
- –
- Schlagworte
- Adenosine Triphosphate - analogs & derivatives, Adenosine Triphosphate - pharmacology, Animals, Animals, Newborn, Biological and medical sciences, Ca 2+ channel, Cadmium - pharmacology, Calcium - metabolism, Calcium - pharmacology, Calcium Channel Blockers - pharmacology, Cells, Cultured, Central nervous system, Dorsomedial hypothalamic neuron, Dose-Response Relationship, Drug, Electrophysiology, Fundamental and applied biological sciences. Psychology, Fura-2 - pharmacokinetics, Fura-2 microfluorometry, Hypothalamus, Middle - cytology, Membrane Potentials - drug effects, Neurons - drug effects, Neurons - physiology, Nystatin-perforated patch-clamp, P2X purinoceptor, Patch-Clamp Techniques, Rats, Rats, Wistar, Vertebrates: nervous system and sense organs