Details

Autor(en) / Beteiligte

• Tan, Xian-Jie
• Lang, Jing-He
• Liu, Dong-Yuan
• Shen, Keng
• Leng, Jin-Hua
• Zhu, Lan

Titel

Expression of vascular endothelial growth factor and thrombospondin-1 mRNA in patients with endometriosis

Ist Teil von

• Fertility and sterility, 2002-07, Vol.78 (1), p.148-153

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective: To investigate the expression of vascular endothelial growth factor (VEGF) mRNA and thrombospondin-1 (TSP-1) mRNA in endometriosis. Design: Molecular studies in human tissue. Setting: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China. Patient(s): Patients undergoing laparoscopy for infertility or other benign gynecologic conditions. Intervention(s): Biopsies were taken from endometriotic lesions (red peritoneal lesion, ovarian endometrioma, and unterosacral ligament nudule) and eutopic endometrium during laparoscopy. Main Outcome Measure(s): mRNA expression from endometriotic lesion and eutopic endometrium was analyzed by reverse transcriptase polymerase chain reaction (PCR) and Northern blotting. Result(s): Among the endometriotic lesions, red peritoneal lesions expressed higher levels of VEGF mRNA and lower levels of TSP-1 mRNA, whereas ovarian endometrioma expressed lower levels of VEGF mRNA and higher levels of TSP-1 mRNA. Eutopic endometrium of women with endometriosis had higher expression levels of VEGF mRNA and lower expression levels of TSP-1 mRNA than that of women without endometriosis. Conclusion(s): The expression of VEGF and TSP-1 in endometriotic lesions appears to be associated with the extent of their neovascularization. The imbalance in expression of VEGF and TSP-1 in the endometrium may play a role in the development of endometriosis.