Details

Autor(en) / Beteiligte

• McColl, Iain H
• Blanch, Ewan W
• Hecht, Lutz
• Kallenbach, Neville R
• Barron, Laurence D

Titel

Vibrational Raman Optical Activity Characterization of Poly(l-proline) II Helix in Alanine Oligopeptides

Ist Teil von

• Journal of the American Chemical Society, 2004-04, Vol.126 (16), p.5076-5077

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2004

Quelle

MEDLINE

Beschreibungen/Notizen

• A vibrational Raman optical activity (ROA) study of a series of alanine peptides in aqueous solution is presented. The seven-alanine peptide Acetyl-OOAAAAAAAOO-Amide (OAO), recently shown by NMR and UVCD to adopt a predominantly poly(l-proline II) (PPII) helical conformation in aqueous solution, gave an ROA spectrum very similar to that of disordered poly(l-glutamic acid) which has long been considered to adopt the PPII conformation, both being dominated by a strong positive extended amide III ROA band at ∼1319 cm-1 together with weak positive amide I ROA intensity at ∼1675 cm-1. A series of alanine peptides Ala2−Ala6 studied in their cationic states in aqueous solution at low pH displayed ROA spectra which steadily evolved toward that of OAO with increasing chain length. As well as confirming that alanine peptides can support the PPII conformation in aqueous solution, our results also confirm the previous ROA band assignments for PPII structure, thereby reinforcing the foundation for ongoing ROA studies of unfolded and partially folded proteins.