Details

Autor(en) / Beteiligte

• Roman, Viviana
• Billard, Christian
• Kern, Catherine
• Ferry‐Dumazet, Hélène
• Izard, Jean‐Claude
• Mohammad, Ramzi
• Mossalayi, Djavad M.
• Kolb, Jean‐Pierre

Titel

Analysis of resveratrol‐induced apoptosis in human B‐cell chronic leukaemia

Ist Teil von

• British journal of haematology, 2002-06, Vol.117 (4), p.842-851

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2002

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Trans‐resveratrol was analysed for its apoptotic and growth inhibitory activity in human B‐cell lines derived from chronic B‐cell malignancies (WSU‐CLL and ESKOL), and in leukaemic lymphocytes from patients with B‐cell chronic lymphocytic leukaemia (B‐CLL). Resveratrol displayed antiproliferative activity on both B‐cell lines, as estimated by the decrease in cell recovery and inhibition of thymidine uptake. Furthermore, resveratrol induced apoptosis in the two cell lines as well as in B‐CLL patients' cells, as evidenced by the increase in annexin V binding, caspase activation, DNA fragmentation and decrease of the mitochondrial transmembrane potential ΔΨm. We previously reported that nitric oxide (NO), endogenously released by an iNO synthase (iNOS) spontaneously expressed in these leukaemic cells, contributed to their resistance towards apoptosis. We show here that resveratrol inhibited both iNOS protein expression and in situ NO release in WSU‐CLL, ESKOL and B‐CLL patients'cells. In addition, Bcl‐2 expression was also inhibited by resveratrol. Thus, downregulation of the two anti‐apoptotic proteins iNOS and Bcl‐2 can contribute to the apoptotic effects of resveratrol in leukaemic B cells from chronic leukaemia. Our data suggest that this drug is of potential interest for the therapy of B‐CLL.