Details

Autor(en) / Beteiligte

• Cynthia S. Brissette-Storkus
• J. C. Kettel
• T. F. Whitham
• K. M. Giezeman-Smits
• L. A. Villa
• D. M. Potter
• William H. Chambers

Titel

Flt-3 ligand (FL) drives differentiation of rat bone marrow-derived dendritic cells expressing OX62 and/or CD161 (NKR-P1)

Ist Teil von

• Journal of leukocyte biology, 2002-06, Vol.71 (6), p.941-949

Ort / Verlag

United States: Society for Leukocyte Biology

Erscheinungsjahr

2002

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Bone marrow‐derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow‐derived rat DC were generated when Flt‐3 ligand (FL) was used as an adjunct to granulocyte macrophage‐colony stimulating factor (GM‐CSF) and interleukin‐4 (IL‐4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen‐presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL‐derived cells, the OX62+ cells were slightly more stimulatory than OX62− cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.