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Strong expression of the rice catalase gene CatB promoter in protoplasts and roots of both a monocot and dicots
Ist Teil von
Plant physiology and biochemistry, 2004-03, Vol.42 (3), p.241-249
Ort / Verlag
Paris: Elsevier Masson SAS
Erscheinungsjahr
2004
Quelle
Elsevier ScienceDirect Journals
Beschreibungen/Notizen
The rice (
Oryza sativa L.) catalase (EC 1.11.1.6) gene
CatB is expressed in roots and cultured cells. We examined the promoter activity of its 5′-flanking region in a monocot and in two dicots. Transient expression assays in rice Oc and tobacco BY-2 suspension cell protoplasts showed that
CatB’s 5′-flanking DNA fragments (nucleotides –1066 to +298) had about 20 and 3–4 times as much promoter activity, respectively, as the CaMV 35S promoter. Serial deletion analyses of the
CatB promoter region revealed that the shortest fragment (–56 to +298) still had about 10 times as much promoter activity as the CaMV 35S promoter in rice protoplasts. In tobacco protoplasts, the activity of the fragment (–56 to +298) was about half of the CaMV 35S promoter. Transgenic rice and
Arabidopsis plants carrying GUS genes driven by the 5′-truncated
CatB promoters were generated and their GUS activity was examined. The region ranging from –329 to +298 showed preferential expression in the roots of rice and
Arabidopsis, and in the shoot apical meristems of
Arabidopsis. In situ hybridization revealed that
CatB was highly expressed in branch root primordia and root apices of rice. Fusion of the GUS gene to the region (–329 to +298) conferred strong expression in these same areas, indicating that the presence of this region was sufficient to express
CatB specifically in the roots. There may be new regulatory element(s) in this region, because it contained no previously known
cis-regulatory elements specific for gene expression in roots.