Neuron (Cambridge, Mass.), 2000-07, Vol.27 (1), p.107-119
2000
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Details
- Autor(en) / Beteiligte
- Titel
- Targeting of PKA to Glutamate Receptors through a MAGUK-AKAP Complex
- Ist Teil von
- Neuron (Cambridge, Mass.), 2000-07, Vol.27 (1), p.107-119
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2000
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Compartmentalization of glutamate receptors with the signaling enzymes that regulate their activity supports synaptic transmission. Two classes of binding proteins organize these complexes: the MAGUK proteins that cluster glutamate receptors and AKAPs that anchor kinases and phosphatases. In this report, we demonstrate that glutamate receptors and PKA are recruited into a macromolecular signaling complex through direct interaction between the MAGUK proteins, PSD-95 and SAP97, and AKAP79/150. The SH3 and GK regions of the MAGUKs mediate binding to the AKAP. Cell-based studies indicate that phosphorylation of AMPA receptors is enhanced by a SAP97-AKAP79 complex that directs PKA to GluR1 via a PDZ domain interaction. As AMPA receptor phosphorylation is implicated in regulating synaptic plasticity, these data suggest that a MAGUK-AKAP complex may be centrally involved.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0896-6273eISSN: 1097-4199DOI: 10.1016/S0896-6273(00)00013-1Titel-ID: cdi_proquest_miscellaneous_71773348
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing, Animals, Binding, Competitive - physiology, Blotting, Western, Carrier Proteins - metabolism, Cyclic AMP-Dependent Protein Kinases - metabolism, Disks Large Homolog 4 Protein, Guanylate Kinases, Hippocampus - cytology, Hippocampus - metabolism, Immunohistochemistry, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Microscopy, Confocal, Nerve Tissue Proteins - physiology, Neurons - metabolism, Nucleoside-Phosphate Kinase - metabolism, Precipitin Tests, Protein Binding - physiology, Rats, Receptors, AMPA - metabolism, Receptors, AMPA - physiology, Receptors, Glutamate - metabolism, Receptors, N-Methyl-D-Aspartate - physiology, Signal Transduction - physiology, Synaptic Transmission - physiology