Biochemical and biophysical research communications, 2004-04, Vol.317 (1), p.54-59
2004
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- Autor(en) / Beteiligte
- Titel
- ERBP, a novel estrogen receptor binding protein enhancing the activity of estrogen receptor
- Ist Teil von
- Biochemical and biophysical research communications, 2004-04, Vol.317 (1), p.54-59
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2004
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- To understand the mechanism by which estrogen receptor (ER) activates transcription in a tissue specific fashion, we isolated ERα binding protein (ERBP) by performing yeast two-hybrid screening with human mammary gland cDNA library. ERBP is a nuclear protein and its mRNA is ubiquitously expressed. The in vitro interaction of ERBP with ERα was demonstrated by GST pull-down assay and this interaction was enhanced by estrogen. In addition, ERBP also bound to PPARγ, RXRα, and ERβ. ERBP interacted with the DNA binding domain and the hinge region of ERα. There are two ERα binding regions on ERBP. The binding of ERBP region at C-terminus to ERα is increased by estrogen while the binding of ERBP region at N-terminus is not affected by estrogen. The interaction of ERBP with ERα was further confirmed in vivo by immunoprecipitation. Transient transfection experiment demonstrated that ERBP enhanced the transcriptional activity of ERα.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/j.bbrc.2004.02.179Titel-ID: cdi_proquest_miscellaneous_71767079
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Line, Cell Nucleus - metabolism, Coactivator, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Estrogen Receptor alpha, Estrogen receptor binding protein, Estrogen receptor α, Humans, Molecular Sequence Data, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Protein Binding, Protein Structure, Tertiary, Receptors, Cytoplasmic and Nuclear - metabolism, Receptors, Estrogen - chemistry, Receptors, Estrogen - metabolism, Receptors, Steroid - metabolism, Recombinant Proteins - genetics, Recombinant Proteins - metabolism, RNA, Messenger - biosynthesis, Sequence Homology, Amino Acid, Tissue Distribution, Transcription Factors - metabolism, Transcriptional Activation, Two-Hybrid System Techniques