Biochemical and biophysical research communications, 2004-04, Vol.316 (3), p.809-815
2004
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- Autor(en) / Beteiligte
- Titel
- Osteopontin as a positive regulator in the osteoclastogenesis of arthritis
- Ist Teil von
- Biochemical and biophysical research communications, 2004-04, Vol.316 (3), p.809-815
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2004
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We examined the role of osteopontin (OPN) in the osteoclastogenesis of arthritis using collagen-induced arthritis (CIA). Cells from arthritic joints of wild-type (OPN +/+) mice spontaneously developed bone-resorbing osteoclast-like cells (OCLs). The cultured cells showed an enhanced expression of receptor activator of nuclear factor κB ligand (RANKL) and a decreased expression of osteoprotegerin (OPG). The addition of OPG reduced the number of OCLs, indicating that the osteoclastogenesis depends on the RANK/RANKL/OPG system. The cells also produced OPN abundantly and anti-OPN neutralizing antibodies suppressed the development of OCLs. Moreover, the addition of OPN increased the expression of RANKL and augmented differentiation of OCLs from OPN-deficient (OPN −/−) cells. OPN, like the combination of 1α,25-dihydroxyvitamin D 3 and dexamethasone, also enhanced the RANKL expression and decreased OPG expression in a stromal cell line, ST2. These results suggest that OPN acts as a positive regulator in the osteoclastogenesis of arthritis through the RANK/RANKL/OPG system.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/j.bbrc.2004.02.124Titel-ID: cdi_proquest_miscellaneous_71737312
- Format
- –
- Schlagworte
- Animals, Arthritis - etiology, Arthritis - metabolism, Calcitriol - metabolism, Carrier Proteins - metabolism, Cell Differentiation, Cell Line, Cells, Cultured, Collagen - metabolism, Collagen-induced arthritis, Dexamethasone - pharmacology, Dose-Response Relationship, Drug, Gene Expression Regulation, Glycoproteins - metabolism, Membrane Glycoproteins - metabolism, Mice, Mice, Inbred DBA, Mice, Transgenic, Models, Genetic, Osteoclast, Osteoclasts - metabolism, Osteopontin, Osteoprotegerin, RANK Ligand, RANKL, Receptor Activator of Nuclear Factor-kappa B, Receptors, Cytoplasmic and Nuclear - metabolism, Receptors, Tumor Necrosis Factor, Recombinant Proteins - chemistry, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - metabolism, Sialoglycoproteins - physiology, Stromal cell, Stromal Cells - cytology