Proteomics (Weinheim), 2004-03, Vol.4 (3), p.609-618
2004
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- Autor(en) / Beteiligte
- Titel
- Photoaptamer arrays applied to multiplexed proteomic analysis
- Ist Teil von
- Proteomics (Weinheim), 2004-03, Vol.4 (3), p.609-618
- Ort / Verlag
- Weinheim: WILEY-VCH Verlag
- Erscheinungsjahr
- 2004
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Multiplexed photoaptamer‐based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal‐to‐noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17‐plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin‐16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1615-9853eISSN: 1615-9861DOI: 10.1002/pmic.200300631Titel-ID: cdi_proquest_miscellaneous_71698780
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Antibodies - chemistry, Aptamers, Biological and medical sciences, Colonic Neoplasms - diagnosis, Colonic Neoplasms - metabolism, Cross-Linking Reagents - chemistry, Cross-Linking Reagents - pharmacology, DNA - chemistry, Dose-Response Relationship, Drug, Endostatins - chemistry, Endostatins - metabolism, Fibroblast Growth Factor 2 - chemistry, Fundamental and applied biological sciences. Psychology, Humans, Hydrogen-Ion Concentration, Interleukin-16 - metabolism, Kinetics, Light, Lod Score, Miscellaneous, Multiplex array, Neoplasm Metastasis, Oligonucleotides - chemistry, Photoaptamer, Protein Array Analysis - instrumentation, Protein Array Analysis - methods, Protein microarray, Proteins, Proteins - chemistry, Proteomics - methods, Tissue Inhibitor of Metalloproteinase-1 - chemistry, Vascular Endothelial Growth Factor A - metabolism