Details

Autor(en) / Beteiligte

• Kim, Tae Yun
• Joo, Il-Joong
• Kang, Shin-Yong
• Cho, Seung-Yull
• Kong, Yoon
• Gan, Xiao-Xian
• Sukomtason, Kom
• Sukomtason, Kabkaew
• Hong, Sung-Jong

Titel

Recombinant Paragonimus westermani Yolk Ferritin Is a Useful Serodiagnostic Antigen

Ist Teil von

• The Journal of infectious diseases, 2002-05, Vol.185 (9), p.1373-1375

Ort / Verlag

Chicago, IL: The University of Chicago Press

Erscheinungsjahr

2002

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• A recombinant protein of Paragonimus westermani yolk ferritin was bacterially produced from a previously cloned complementary DNA and was used as an antigen for an enzyme-linked immunosorbent assay (ELISA) against paragonimiasis- and other helminth-infected sera to evaluate its serodiagnostic potential. The ELISA revealed that paragonimiasis westermani had 88.2%sensitivity and 100% specificity. The positive and negative predictive values of the ELISA were calculated to be 100% and 97.1%, respectively. Sera from cats experimentally infected with P. westermani began to produce immunoglobulin G antibodies against the yolk ferritin at 13 weeks after infection, which suggests that the corresponding antigen was derived from the vitellaria in accordance with maturation of P. westermani. These results indicate that the recombinant P. westermani yolk ferritin is a potent serodiagnostic reagent for paragonimiasis westermani from an early stage of the infection.