Details

Autor(en) / Beteiligte

• Miki, Akihiro
• Tatsuno, Michiaki
• Katagi, Munehiro
• Nishikawa, Mayumi
• Tsuchihashi, Hitoshi

Titel

Simultaneous Determination of Eleven Benzodiazepine Hypnotics and Eleven Relevant Metabolites in Urine by Column-Switching Liquid Chromatography-Mass Spectrometry

Ist Teil von

• Journal of analytical toxicology, 2002-03, Vol.26 (2), p.87-93

Ort / Verlag

Niles, IL: Oxford University Press

Erscheinungsjahr

2002

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase extraction technique was employed to enhance sensitivity and eliminate tedious sample pretreatment. The combination of an N-vinylacetamide-containing hydrophilic polymer online extraction column and a C18 semi-micro LC column provided the successful separations and MS determinations of the 11 benzodiazepines and 11 relevant metabolites tested in this study. The detection limits ranged 2–10 ng/mL and 10–50 ng/mL in the SIM and full-scan modes, respectively, using 50 µL of urine. The calibration curves were linear up to 500 ng/ml, for all these analytes in the SIM mode. The present method provided successful forensic applications for the proof of benzodiazepine administration.