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Developmental changes in the protein composition of sphingolipid- and cholesterol-enriched membrane domains of rat cerebellar granule cells
Journal of neuroscience research, 2002-03, Vol.67 (6), p.729-738
Palestini, Paola
Botto, Laura
Guzzi, Francesca
Calvi, Chiara
Ravasi, Daniela
Masserini, Massimo
Pitto, Marina
2002
Details
Autor(en) / Beteiligte
Palestini, Paola
Botto, Laura
Guzzi, Francesca
Calvi, Chiara
Ravasi, Daniela
Masserini, Massimo
Pitto, Marina
Titel
Developmental changes in the protein composition of sphingolipid- and cholesterol-enriched membrane domains of rat cerebellar granule cells
Ist Teil von
Journal of neuroscience research, 2002-03, Vol.67 (6), p.729-738
Ort / Verlag
New York: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2002
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
The biological role of cell membrane domains has been investigated in a number of eukariotic cells, but less attention has been paid to the neuron. In the present investigation, we assessed the changes in lipid and protein composition of detergent‐resistant membrane fractions prepared from cultured rat cerebellar granule cells, during differentiation and maturation in vitro. At any stage of the cell life, low‐density, detergent‐resistant fractions, characterised by the specific presence of prion protein, were enriched in glycolipids, cholesterol, and sphingomyelin. The enrichment in sphingomyelin was developmentally regulated, increasing continuously during cell differentiation and maturation. Concerning proteins, domains were enriched in Fyn and TAG‐1, which present exclusively within this fraction at any stage of cell culture, and in GAP‐43, mainly during the differentiation stage. On the other side, proteins affecting signal transduction and cytoskeleton‐related proteins (heterotrimeric G‐proteins, protein kinase C, MARCKS, tubulin), were not enriched within detergent‐resistant fractions during cell differentiation, but were recovered within this fraction in mature neurons. These results indicate that during different cellular life stages, specific proteins are recruited within detergent‐resistant membrane domains of the neuron and suggest their involvement in specific physiological phenomena (differentiation, maturation and/or aging). © 2002 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0360-4012
eISSN: 1097-4547
DOI: 10.1002/jnr.10170
Titel-ID: cdi_proquest_miscellaneous_71510078
Format
–
Schlagworte
Animals
,
Cell Adhesion Molecules, Neuronal - analysis
,
Cell Differentiation - physiology
,
Cell Fractionation
,
Cellular Senescence - physiology
,
cerebellar granule cells
,
Cerebellum - cytology
,
Contactin 2
,
detergent-resistant fractions
,
Detergents
,
differentiation
,
Electrophoresis, Gel, Two-Dimensional
,
GAP-43 Protein - analysis
,
glycolipids
,
membrane domains
,
Membrane Microdomains - chemistry
,
Membrane Microdomains - metabolism
,
Neurons - chemistry
,
Neurons - cytology
,
Neurons - metabolism
,
Protein Kinase C - analysis
,
Rats
,
Rats, Sprague-Dawley
,
Tubulin - analysis
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