Details

Autor(en) / Beteiligte

• van der Zander, Kim
• Houben, Alphons J. H. M
• Kroon, Abraham A
• De Mey, Jo G. R
• Smits, Paul A. B. M
• de Leeuw, Peter W

Titel

Nitric oxide and potassium channels are involved in brain natriuretic peptide induced vasodilatation in man

Ist Teil von

• Journal of hypertension, 2002-03, Vol.20 (3), p.493-499

Ort / Verlag

Hagerstown, MD: Lippincott Williams & Wilkins, Inc

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• OBJECTIVE Brain natriuretic peptide (BNP) causes vasodilatation but the mechanisms by which this is accomplished are not fully known. The aim of the present study was to determine whether, besides KCa2+-channels, nitric oxide (NO) is involved in BNP-induced vasodilatation. METHODS We studied 10 healthy males twice, in random order, at an interval of 2 weeks. Experiments always started with infusion of BNP (8–16–32–64 pmol/dl per min) into the brachial artery. On the first day this infusion was followed by a second BNP infusion combined with the KCa2+-channel-blocker, tetraethylammonium (TEA, 0.1 mg/dl per min), and on the other day by BNP infusion combined with the NO-synthase inhibitor, l-N-monomethyl arginine (l-NMMA, 0.8 μmol/dl per min). The latter was then followed by a combined infusion of BNP, l-NMMA and TEA. All infusions were separated by a 1 h washout period. Forearm blood flow (FBF) was determined by venous occlusion plethysmography. RESULTS Mean arterial pressure and heart rate did not change during any of the experiments. BNP alone induced a dose-dependent dilatation, which was similar on both days. TEA, l-NMMA, and their combination all reduced the BNP-induced dilatation (P < 0.05). The combined infusion had a significantly greater effect than TEA alone (P = 0.005). BNP infusions were associated with a significant increase in plasma cyclic guanosine monophosphate (cGMP) and C-type natriuretic peptide (CNP) (P < 0.05). CONCLUSIONS BNP induces arterial vasodilatation not only by opening KCa2+-channels, but also via stimulation of NO production. In addition, BNP stimulates net CNP increase.