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Improving typeability of multiple bacterial species using pulsed-field gel electrophoresis and thiourea
Ist Teil von
Diagnostic microbiology and infectious disease, 2003-12, Vol.47 (4), p.619-621
Ort / Verlag
New York, NY: Elsevier Inc
Erscheinungsjahr
2003
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Although pulsed-field gel electrophoresis is considered the “gold standard” technique for molecular typing, typeability may not be excellent for some bacterial species because of DNA degradation. Previous reports suggest that the addition of thiourea in the gel buffer can improved the typeability for some species. In the present study, 66 Gram-negative strains (seven species) known to be affected by DNA degradation and four control strains were evaluated by PFGE with and without the addition of 50 μg/M of thiourea to the buffer used in the electrophoresis. Macrorestriction patterns were obtained for all
K. pneumoniae,
S. marcescens,
P. aeruginosa, and
Salmonella spp., for 95.4% of
E. coli, and for 50% of
E. cloacae strains from the gels performed in the buffer with throurea. However, typeability was not improved for
Acinetobacter spp. The range of non-typeable species for which thiourea can limit the problem of DNA degradation is considerably wider than described in previous publications.