The EMBO journal, 2002-03, Vol.21 (5), p.1012-1020
2002
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Details
- Autor(en) / Beteiligte
- Titel
- The C. elegans ric-3 gene is required for maturation of nicotinic acetylcholine receptors
- Ist Teil von
- The EMBO journal, 2002-03, Vol.21 (5), p.1012-1020
- Ort / Verlag
- England
- Erscheinungsjahr
- 2002
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- Mutations in ric-3 (resistant to inhibitors of cholinesterase) suppress the neuronal degenerations caused by a gain of function mutation in the Caenorhabditis elegans DEG-3 acetylcholine receptor. RIC-3 is a novel protein with two transmembrane domains and extensive coiled-coil domains. It is expressed in both muscles and neurons, and the protein is concentrated within the cell bodies. We demonstrate that RIC-3 is required for the function of at least four nicotinic acetylcholine receptors. However, GABA and glutamate receptors expressed in the same cells are unaffected. In ric-3 mutants, the DEG-3 receptor accumulates in the cell body instead of in the cell processes. Moreover, co-expression of ric-3 in Xenopus laevis oocytes enhances the activity of the C.elegans DEG-3/DES-2 and of the rat alpha-7 acetylcholine receptors. Together, these data suggest that RIC-3 is specifically required for the maturation of acetylcholine receptors.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0261-4189DOI: 10.1093/emboj/21.5.1012Titel-ID: cdi_proquest_miscellaneous_71494799
- Format
- –
- Schlagworte
- Acetylcholine - pharmacology, alpha7 Nicotinic Acetylcholine Receptor, Amino Acid Sequence, Animals, Caenorhabditis elegans, Caenorhabditis elegans - genetics, Caenorhabditis elegans - metabolism, Caenorhabditis elegans Proteins - chemistry, Caenorhabditis elegans Proteins - genetics, Caenorhabditis elegans Proteins - physiology, Cell Death, DEG-3 protein, Drosophila Proteins - chemistry, Drug Resistance, Freshwater, Genes, Helminth, Levamisole - pharmacology, Molecular Sequence Data, Muscle Proteins - chemistry, Muscle Proteins - genetics, Muscle Proteins - physiology, Nerve Tissue Proteins - chemistry, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - physiology, Neurons - metabolism, Oocytes, Protein Structure, Tertiary, Protein Subunits, Protein Transport, Rats, Receptors, GABA - metabolism, Receptors, Glutamate - metabolism, Receptors, Nicotinic - genetics, Receptors, Nicotinic - physiology, Recombinant Fusion Proteins - physiology, ric-3 gene, RIC-3 protein, Sequence Alignment, Sequence Homology, Amino Acid, Synaptic Transmission - physiology, Xenopus laevis