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Screening of blood from potential organ and cornea donors for viruses
Journal of medical virology, 2002-04, Vol.66 (4), p.571-575
Miédougé, Marcel
Chatelut, Martine
Mansuy, Jean-Michel
Rostaing, Lionel
Malecaze, François
Sandres-Sauné, Karine
Boudet, Francis
Puel, Jacqueline
Abbal, Michel
Izopet, Jacques
2002
Details
Autor(en) / Beteiligte
Miédougé, Marcel
Chatelut, Martine
Mansuy, Jean-Michel
Rostaing, Lionel
Malecaze, François
Sandres-Sauné, Karine
Boudet, Francis
Puel, Jacqueline
Abbal, Michel
Izopet, Jacques
Titel
Screening of blood from potential organ and cornea donors for viruses
Ist Teil von
Journal of medical virology, 2002-04, Vol.66 (4), p.571-575
Ort / Verlag
New York: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2002
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Prospective nucleic acid tests were carried out for human immunodeficiency virus (HIV) and hepatitis C virus (HCV) using the COBAS Amplicor HIV‐1 and HCV tests (Roche Diagnostics, Meylan, France) on potential organ (n = 113) and cornea (n = 368) donors in France to evaluate their performance and suitability for use as a complement to routine serological tests. Blood samples were collected from organ donors with preserved cardiac function after verification of cerebral death. Blood samples were collected from cornea donors post‐mortem within 48 hr after death. An internal control was added to the samples before extraction to monitor each individual polymerase chain reaction (PCR). The nucleic acid tests were always interpretable in organ donors and negative in all except in 2 anti‐HCV positive patients. One had an indeterminate HIV p24 antigen but was negative for HIV RNA. HIV and HCV RNA were not found in cornea donors with a negative serology but indeterminate molecular results were frequent in this group (17.6%). Cornea donors also gave significantly more (14.4%) indeterminate serological results than organ donors (1.8%) (P < 0.001). This was due to the poor quality of the blood samples collected post‐mortem. However, there was no correlation between indeterminate results of serological and molecular tests. There were 16/19 (84%) indeterminate serological results for HIV and 4/4 (100%) for HCV that were negative by PCR. Thus, nucleic acid tests could be useful for qualifying a donor whose serological results are indeterminate. The extraction procedures on post‐mortem specimens and/or blood collection must be changed to improve the performance of nucleic acid tests. J. Med. Virol. 66:571–575, 2002. © 2002 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0146-6615
eISSN: 1096-9071
DOI: 10.1002/jmv.2183
Titel-ID: cdi_proquest_miscellaneous_71468595
Format
–
Schlagworte
Biological and medical sciences
,
Cornea - virology
,
cornea donors
,
France
,
Fundamental and applied biological sciences. Psychology
,
HCV
,
Hepacivirus - isolation & purification
,
Hepatitis C - virology
,
Hepatitis C virus
,
HIV
,
HIV Infections - virology
,
HIV-1 - isolation & purification
,
Human immunodeficiency virus 1
,
Humans
,
Microbiology
,
Nucleic Acid Amplification Techniques
,
nucleic acid testing
,
organ donors
,
Organ Transplantation
,
Reagent Kits, Diagnostic
,
RNA, Viral - blood
,
Tissue Donors
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