Details

Autor(en) / Beteiligte

• Yoshino, Osamu
• Osuga, Yutaka
• Koga, Kaori
• Hirota, Yasushi
• Yano, Tetsu
• Tsutsumi, Osamu
• Fujimoto, Akihisa
• Kugu, Koji
• Momoeda, Mikio
• Fujiwara, Toshihiro
• Taketani, Yuji

Titel

Upregulation of Interleukin-8 by Hypoxia in Human Ovaries

Ist Teil von

• American journal of reproductive immunology (1989), 2003-10, Vol.50 (4), p.286-290

Ort / Verlag

Oxford, UK: Munksgaard International Publishers

Erscheinungsjahr

2003

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• Problem: To evaluate the effect of hypoxia on interleukin (IL)‐8 expression in human ovarian follicles. Method of study: Follicular fluid (FF) from each follicle was separately collected from women undergoing in vitro fertilization and embryo transfer. Concentrations of oxygen, progesterone, estradiol, IL‐1α/β, IL‐8, and tumor necrosis factor (TNF)‐α in FF were measured. Isolated granulosa‐lutein cells (GLC) from obtained FF were cultured under normoxic or hypoxic conditions, and concentrations of IL‐8 in culture media were measured. Results: Simple regression analysis demonstrated a significant negative correlation between the concentrations of IL‐8 and oxygen in FF (r = 0.50, P < 0.0001). However, none of the concentrations of progesterone, estradiol, IL‐1β, and TNF‐α in FF showed a significant correlation with IL‐8 concentrations. Hypoxia stimulated the secretion of IL‐8 by cultured GLC over twofolds compared with a normoxic control (P < 0.05). Conclusions: These findings suggest that IL‐8, like other angiogenic factors, is upregulated under hypoxic condition, which argues that hypoxia in the ovarian follicles comes into play in ovarian functions by inducing a range of proangiogenic and chemoattractive substances.