Details

Autor(en) / Beteiligte

• Weind, K.L.
• Ellis, C.G.
• Boughner, D.R.

Titel

Aortic valve cusp vessel density: Relationship with tissue thickness

Ist Teil von

• The Journal of thoracic and cardiovascular surgery, 2002-02, Vol.123 (2), p.333-340

Ort / Verlag

Philadelphia, PA: Elsevier Inc

Erscheinungsjahr

2002

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Objectives: The presence of a microvasculature within aortic cusps implies that tissue oxygen requirements exceed the amount deliverable by diffusion from the tissue surfaces alone. For the design of a successful tissue-engineered valve replacement, the effect of diffusion distance (tissue thickness) on oxygen delivery must be considered. We therefore examined in normal aortic valve cusps the relationship between the presence of microvessels and the tissue thickness. Methods: Thirty porcine aortic valve cusps were excised and examined after cusp microvessels were pressure filled with a carbon particle solution. Cusp images were captured for stereographic vessel density analysis, and cusp thickness was determined with a radiographic technique. Histologic cross-sections were evaluated to determine vessel depth from the cusp surface. Results: Cusp basal regions measured 0.69 to 0.86 mm in thickness, significantly thicker (P = .001) than the rest of the cusp, which measured 0.36 to 0.48 mm. In general a vascular bed was present when cusp thickness exceeded 0.5 mm, with a median value of 5.16 vessels/mm3. Conclusions: From published values of arterial wall oxygen consumption and diffusivity, we predicted that the probable maximum oxygen diffusion distance for valve tissue would be about 0.2 mm. This was consistent with our physical findings, which implies that central tissue anoxia is avoided by the capillary bed. An avascular tissue-engineered valve metabolically similar to an aortic valve should therefore not exceed a thickness of approximately 0.40 mm.