Details

Autor(en) / Beteiligte

• Yoshikawa, H.
• Tajiri, Y.
• Sako, Y.
• Hashimoto, T.
• Umeda, F.
• Nawata, H.

Titel

Effects of biotin on glucotoxicity or lipotoxicity in rat pancreatic islets

Ist Teil von

• Metabolism, clinical and experimental, 2002-02, Vol.51 (2), p.163-168

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2002

Quelle

EZB-NALI5-00465 Elsevier Archive NL

Beschreibungen/Notizen

• Biotin (vitamin H) plays an important role as a cofactor in glucose or lipid metabolism. We showed that biotin potentiated glucose-induced insulin release in isolated rat islets, while biotin alone did not affect insulin release. Coculture with biotin in islets for 48 hours significantly enhanced glucose-induced insulin release or islet insulin content. Similarly, preproinsulin or pancreatic/duodenal homeobox-1 (PDX-1) mRNA was also enhanced in islets cultured with biotin for 48 hours. Furthermore, we measured effects of biotin on [beta ]-cell function under glucotoxic or lipotoxic states. In islets cultured with high glucose or palmitate for 48 hours, glucose-induced insulin release or islet insulin content deteriorated. Coculture with biotin significantly restored glucose-induced insulin release or islet insulin content together with the restoration of preproinsulin or PDX-1 mRNA. We conclude that biotin exerts its beneficial effects on [beta ]-cell dysfunction induced by glucose or free fatty acids probably through the enhancement of insulin biosynthesis.