Details

Autor(en) / Beteiligte

• SEUNG MYUNG DONG
• PAI, Sara I
• SIDRANSKY, David
• RHA, Seo-Hee
• HILDESHEIM, Allan
• KURMAN, Robert J
• SCHWARTZ, Peter E
• MORTEL, Rodrigue
• MCGOWAN, Larry
• GREENBERG, Mitchell D
• BARNES, Willard A

Titel

Detection and Quantitation of Human Papillomavirus DNA in the Plasma of Patients with Cervical Carcinoma

Ist Teil von

• Cancer epidemiology, biomarkers & prevention, 2002-01, Vol.11 (1), p.3-6

Ort / Verlag

Philadelphia, PA: American Association for Cancer Research

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• Human papillomaviruses (HPVs) play a central role in the development of cervical carcinoma. Plasma DNA from 232 patients taken at diagnosis or after treatment for invasive cervical cancer ( n = 175) or carcinoma in situ ( n = 57) and 60 normal controls were examined for HPV-16 or HPV-18 E7 DNA by conventional and real-time quantitative PCR assays. We found HPV-16 or HPV-18 E7 DNA in 6.9% (11 of 175) of invasive cervical cancer cases (18.1% of cases positive for HPV-16 or HPV-18 at the genital tract), 1.8% (1 of 57) of carcinoma in situ , and 1.7% (1 of 60) of normal controls by conventional PCR. Quantitative PCR identified the highest concentrations of HPV DNA (copy number of HPV/ml of plasma) in patients with invasive cervical cancer (mean, 11,163; median, 183.5), followed by a level of 8 in the single carcinoma in situ case and 0 copies in the normal control initially positive by conventional PCR. HPV DNA can be detected in the plasma of some patients with HPV-positive cervical tumors. It remains to be demonstrated whether quantitative PCR analysis of HPV DNA in plasma may have utility in patients at high risk of recurrent disease.