Details

Autor(en) / Beteiligte

• Lenaz, Giorgio
• Paolucci, Ugo
• Fato, Romana
• D'Aurelio, Marilena
• Parenti Castelli, Giovanna
• Sgarbi, Gianluca
• Biagini, Graziella
• Ragni, Luca
• Salardi, Silvana
• Cacciari, Emanuele

Titel

Enhanced Activity of the Plasma Membrane Oxidoreductase in Circulating Lymphocytes from Insulin-Dependent Diabetes Mellitus Patients

Ist Teil von

• Biochemical and biophysical research communications, 2002-02, Vol.290 (5), p.1589-1592

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2002

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Circulating human lymphocytes contain a transmembrane oxidoreductase (PMOR) capable of reducing dichlorophenol indophenol (DCIP) by endogenous reductants, presumably NADH. Membranes from lymphocytes obtained from buffy coats contain a NADH DCIP reductase having a Km of about 1 μM and almost insensible to dicoumarol. The PMOR of lymphocytes from insulin-dependent diabetic patients is higher than that from age-matched controls and, in addition, has a dicoumarol-sensitive component, lacking in most controls, presumably due to membrane association of DT-diaphorase. The increase of PMOR in diabetes is likely due to overexpression of the enzyme, in view of the very low Km for NADH indicating that, in intact cells, the enzyme is practically saturated with the reductant substrate.