Molecular carcinogenesis, 2003-12, Vol.38 (4), p.179-187
2003
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- Autor(en) / Beteiligte
- Titel
- Sequestration of connexin43 in the early endosomes: An early event of Leydig cell tumor progression
- Ist Teil von
- Molecular carcinogenesis, 2003-12, Vol.38 (4), p.179-187
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2003
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Connexins form gap junction channels that allow intercellular communication between neighboring cells. Compelling evidence has revealed that Cx are tumor‐suppressor genes and reduced Cx expression has been related with uncontrolled cell growth in tumors and transformed cells. In the present study, we addressed Cx transcriptional and posttranscriptional regulations during the earlier stage of testicular tumors confined to Leydig cells in a transgenic mice model. In situ hybridization indicated that connexin43 (Cx43) mRNA was highly expressed either at early tumorogenesis (3 m) characterized by intense proliferation of Leydig cells, or at advanced tumorogenesis (6–7 m) when tumor cells completely invaded the testis. In contrast, Cx43 protein analyzed by Western blotting or classic immunohistochemical analyses was present at the beginning of tumor progression, but was dramatically reduced as tumor advanced. Application of high‐resolution deconvolution microscopy to testis sections demonstrates that cells that proliferate exhibited an aberrant cytoplasmic Cx43 localization, in contrast to the expected plasma membrane Cx43 localization in normal Leydig cells. Dual immunofluorescence labeling with specific markers of cellular compartments shows that cytoplasmic Cx43 signal was mainly sequestered within early endosomes. Altogether, this study provides the first evidence that impaired Cx43 trafficking in endosomes is an early event associated with uncontrolled cell proliferation that could serve as a neoplastic marker. © 2003 Wiley‐Liss, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0899-1987eISSN: 1098-2744DOI: 10.1002/mc.10160Titel-ID: cdi_proquest_miscellaneous_71390708
- Format
- –
- Schlagworte
- Animals, Cell Membrane, Connexin 43 - genetics, Connexin 43 - metabolism, Cx43 sequestration, Disease Progression, early endosomes, endosomes, Endosomes - metabolism, Fluorescent Antibody Technique, Gap Junctions - ultrastructure, Gene Expression Regulation, Neoplastic, Immunoenzyme Techniques, In Situ Hybridization, Inhibins - deficiency, Inhibins - genetics, Inhibins - metabolism, Leydig Cell Tumor - genetics, Leydig Cell Tumor - metabolism, Leydig Cell Tumor - pathology, Leydig cell tumors, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Protein Transport, RNA, Messenger - genetics, RNA, Messenger - metabolism