Analytical biochemistry, 2002-01, Vol.300 (2), p.139-145
2002
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Details
- Autor(en) / Beteiligte
- Titel
- A Preservation Method That Allows Recovery of Intact RNA from Tissues Dissected by Laser Capture Microdissection
- Ist Teil von
- Analytical biochemistry, 2002-01, Vol.300 (2), p.139-145
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2002
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1006/abio.2001.5463Titel-ID: cdi_proquest_miscellaneous_71349567
- Format
- –
- Schlagworte
- Animals, Blotting, Northern, Cryopreservation - methods, Dissection - methods, Embryo, Mammalian - cytology, Embryo, Mammalian - metabolism, Humans, laser capture microdissection, Lasers, Mice, Polymerase Chain Reaction, RNA - genetics, RNA - isolation & purification, RNA - metabolism, RNA, Messenger - genetics, RNA, Messenger - isolation & purification, RNA, Messenger - metabolism, Sucrose, Thyroid Neoplasms - genetics, Thyroid Neoplasms - pathology, Tissue Preservation - methods